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TEST #
1 with CHITOSAN |
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Seedlings
of SYNSEPALUM DULCIFICUM have grown 4 weeks in a light soil mixed with coco
fiber
Synsepalum are hard to grow after they have
been prepared for export and traveled several days bare roots
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After
washing out the roots with water ( a gently dip suffices, no soil particles
are firmly attached ), 5 plantlets are dipped in our CHITOSAN solution
and 5 are not. The two groups are wrapped in the same dry paper
3 days, our
temperature being 25 - 33 Celsius with high humidity : such conditions of
heat and lack of humidity put much stress on the plantlets, especially the
lack of humidity of the wrapping paper. On day 2, by
applying the hands on the paper we clearly feel that much less
evaporation has occurred from the plants treated with CHITOSAN .
After
day 3 the two groups are separately grown under plastic sheet in a a pot.
Until day 4 it looks like all plantlets will survive. But on day 5, one of
the non treated plantlets die. On day 6 the results are shown below : the
group of the 5 untreated plantlets on the right side have not dried out yet
but they are obviously on the verge of dying. On the left side ( a piece of
bark separates the 2 sides ) among the 5 CHITOSAN treated plantlets, 2 are in a bad
shape and 3 seem to have recovered definitely. |
On day
7 the result are as follows : the 5 non treated plantlets have died while in
the 5 CHITOSAN treated plantlets ( with a yellow point below ), 3 have
recovered. |
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TEST #
2 with CHITOSAN |
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Here the idea is to test 20 Synsepalum sprayed with CHITOSAN a week before packing and see how they
react to different packing conditions. The plants are 40 cm high and about 2 years
old. |
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They were grown in a light soil mixed
with husk of rice, both burnt ( black ) and not burnt. Clearly the soil
adheres very loosely to the roots : it is a very well drained mix, not
sticky and slightly acidic ( pH 6.7 ). |
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In a tank of clean water ( not
saturated with dirt ) |
the roots will be washed out
most easily |
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However dipping in clear water
is not efficient if the plants must pass through strict phytosanitary control : many
bits of soil and dirt are still attached to the roots ( the pink points
below ); using hands to withdraw them would stress the roots and take time;
whereas at this stage speed is the key to a good survival rate. |
In order to avoid delaying the
wrapping of the plants we use a fine mist of water under
pressure to remove all the attached soil particles |
The 20 Synsepalum are all dipped in pesticides
and fungicide, then divided into 4 groups to test the effects of using
CHITOSAN dipping and PLASTIC BAG for a 3 days stay in cartons.
# 1 : CHITOSAN + PLASTIC BAG # 2 :
CHITOSAN NO PLASTIC BAG # 3 : NO CHITOSAN +
PLASTIC BAG # 4 : NO
CHITOSAN NO PLASTIC BAG |
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Plant gel and moist paper will prevent the roots from drying out
during 3 days in the carton |
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On day 4 the 4 groups are unpacked and potted in a well drained soil
mixed with coco bits , rice husk and charcoal. |
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From day 4 to day 10 the 20 plants are put with
their pots under close plastic bags. ( a single big bag for several plants
). Our temperature ( we are in the rainy season ) is from 25 Celsius at
night to 33 daytime. It rains long hours almost every day outside the
bags. |
On day 11 the plastic bags are open. We don't
have the contrasted results of our test # 1 above with the plantlets
lacking moisture during 3 days in cartons; in this test # 2 the plants
without CHITOSAN dipping look slightly more stressed, but all of the 20 plants are in
a very good shape, whatever be their group. Based on the results of our test
# 1 we link this good shape to the spraying of CHITOSAN several days before
packing. |
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TEST #
3 with CHITOSAN |
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In this test we have dipped two groups
( small size and big size )
of CODARIOCALYX MOTORIUS
in in our
CHITOSAN solution. After 5 days in
cartons, the leaves of the 2 groups are all down but some small white roots have grown (
they can be seen on the picture below ). We repot them in a light soil. |
After 10 days in soil covered with plastic bags ( hence without watering )
at outside temperature the survival rate is 100 %. Indeed
CODARIOCALYX MOTORIUS plants in the 2
groups are doing well, with many new
small leaves. Given the very fine stems of these plants, and their
apparently fragile structure, plus 5 days in cartons at tropical
temperature, a 100 % survival rate is quite noticeable. |
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TEST #
4 with CHITOSAN
on FRUIT PLANTS
( please right - click ) |
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